https://dspace.univ-ouargla.dz/jspui/handle/123456789/13144 Tue, 21 Apr 2026 17:11:34 GMT 2026-04-21T17:11:34Z https://dspace.univ-ouargla.dz/jspui/handle/123456789/13147 Titre: Planomonospora sp. PM18: ISOLATION AND TAXONOMY OF NEW ACTINOBACTERIAL STRAIN ISOLATED FROM ALGERIAN SAHARAN SOIL Auteur(s): CHAABANE CHAOUCH Fawzia; BOUZNADA Khaoula; BOURAS Noureddine; TATA Samira; MEKLAT Atika; MOKRANE Salim; LAMARI Lynda; KEMASSI Abdellah; MATHIEU Florence; SABAOU Nasserdine Résumé: An actinobacterial strain, designated PM18, was isolated from a Saharan soil sample by agar plate dilution method on chitin-vitamin agar supplemented with polymyxin after pretreatment of the soil sample at 120°C (dry heat) for 1 h. The taxonomic status of this strain was determined basis on morphological and physiological characteristics and phylogenetic analysis based on the 16S rRNA gene sequence. Strain PM18 developed an extensively branched and non-fragmented substrate mycelium. The isolate was characterized by the presence of cylindrical monosporous sporangia, which were produced only on the aerial mycelium. Sporangia were arranged closely in parallel double rows. The monosporous sporangia were motile. The morphological features of this isolate corresponded to those of members of the genus Planomonospora. Furthermore, phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain PM18 is a member of the genus Planomonospora. The 16S rRNA gene sequence similarity showed that this strain was most closely related to Planomonospora sphaerica JCM 9374T (99.2%) and Planomonospora parontospora subsp. antibiotica JCM 3094T (98.8%). Based on phenotypic differences and the separate position of strain PM18 in the phylogenetic tree, this strain may be a representative of a putative novel species in the genus Planomonospora. Description: Algerian Journal of Arid And Environment (AJAE) Thu, 01 Dec 2016 00:00:00 GMT https://dspace.univ-ouargla.dz/jspui/handle/123456789/13147 2016-12-01T00:00:00Z https://dspace.univ-ouargla.dz/jspui/handle/123456789/13155 Titre: LE PETIT RENARD DES HAUTES PLAINES DE TIARET: UNE ESPECE MECONNUE EN DANGER! Auteur(s): BENCHOHRA Mokhtar; AMARA Karim Résumé: In Algeria, the fox (Vulpes) is still remains a not well known wild species. Moreover, the small leaving in the highland of Tiaret is not mentioned in any report or a published study. This study presents monitored cases of dead fox in the roads and trapped by villagers, in Tiaret’s East area. This small fox seem to be native to this area; however, a considerable risk threatens its existence. When the fox dens were closer to human habitation, it increases trapping risk, foxes are frequently strucked by cars in roads, and stray and roaming dog populations may be the most hazard, and serious limiting factors to this endangered fox expansion. Consequently, serious studies must be conducted to recognition this vulpes species and its conservation. Description: Algerian Journal of Arid And Environment (AJAE) Thu, 01 Dec 2016 00:00:00 GMT https://dspace.univ-ouargla.dz/jspui/handle/123456789/13155 2016-12-01T00:00:00Z https://dspace.univ-ouargla.dz/jspui/handle/123456789/13148 Titre: COMPARAISON DE L’ACTIVITE PROTEASIQUE DES SOUCHES DE Bacillus thuringiensis STRAINS Auteur(s): SELLAMI Sameh; ABDELKEFI-MESRATI Lobna; TOUNSI Slim; KAIS Jamoussi Résumé: During this work, a screening among 4 receiving host cells of B. thuringiensis was realized in order to distinguish the least producing proteolytic enzymes strain. We found that the proteolytic activity of the B. thuringiensis subspecies israelensis 14T and 4Q7 after 48 hours is about 165.9 IU and at 138.08 IU, respectively. While the B. thuringiensis subspecies kurstaki HD1cryB and the cured BUPM95 is about 95.36 IU and 400.62 IU, respectively. Therefore, HD1cryB and 4Q7 were chosen as hosts cells for the pHT-Blue-vip3Aa16 plasmid. The electroporation of B. thuringiensis strains was verified via the PCR amplification since a band of 820 pb corresponding to the vip3Aa16 gene was detected. The synthesis of the protein Vip3Aa16 from the recombinant strains HD1cryB (pHT-Blue-vip3Aa16) and 4Q7 (pHT-Blue-vip3Aa16) demonstrated that HD1cryB proteases did not affect the Vip3Aa16 expression rate. Thus, the B. thuringiensis HD1cryB could be a very suitable host strain. Description: Algerian Journal of Arid And Environment (AJAE) Thu, 01 Dec 2016 00:00:00 GMT https://dspace.univ-ouargla.dz/jspui/handle/123456789/13148 2016-12-01T00:00:00Z https://dspace.univ-ouargla.dz/jspui/handle/123456789/13152 Titre: EFFET DU STRESS SALIN SUR LA VARIATION D’ISOENZYMES D'ESTERASES DE DEUX GENOTYPES CONTRASTES DE MEDICAGO Auteur(s): AMOURI Adel Amar Résumé: The activity of the esterases wich is related to the physiological and metabolic stage of the plant cell, can be affected by the environmental factors such as the salt stress. Our study was carried on the native electrophoresis of the esterases isoenzymes, on two ecotypes from two different species of Medicago, the tolerant from M. truncatula Gaertn and the sensitive one from M. polymorpha L. The study of the synthesis of esterases isozymes during germination of seeds under different salt concentration of salinity 0, 68, 102 and 137 mM, showed a specific quantitative and qualitative variations of isoenymes synthesis, between the two contrasting. This biochemical polymorphism of esterases isoenzymes, can be explained by the genetic polymorphism of genes involved in salt stress tolerance in Medicago. Description: Algerian Journal of Arid And Environment (AJAE) Thu, 01 Dec 2016 00:00:00 GMT https://dspace.univ-ouargla.dz/jspui/handle/123456789/13152 2016-12-01T00:00:00Z