EXTRACTION AND PRELIMINARY CHARACTERIZATION OF ALKALI-SOLUBLE POLYSACCHARIDES FROM Plantago ciliata DESF. SEEDS

Abstract

Extraction of crude alkali-soluble polysaccharides from Plantago ciliata seeds, using hot alkaline solution KOH at 80°C for 2h generate tow fractions GPSAI and GPSAII. The mass yield of each one is 3.63% and 3.19% (w/w), respectively refering to the dry weight of the seeds. They are contained 43.77±0.19, 73.77±0.17% neutral sugars, 0.00, 21.95±0.07% uronic acid and 10.42±0.01, 10.54±0.01% protein, for GPSAI and GPSAII respectively. Crude alkali-soluble polysaccharides consist of an hetero-neutral polysaccharides. It was mainly composed of arabinose and xylose in addition to the traces of galactose using Thin Layer Chromatography analysis.