MORPHOLOGICAL CHARACTERIZATION AND PRELIMINARY SCREENING FOR ANTIBACTERIAL ACTIVITY OF ACTINOBACTERIA STRAINS ISOLATED FROM TAMANRASSET SOIL AS A HYPER ARID REGION IN SOUTHERN ALGERIA

Abstract

In this study, we aimed to isolate Actinobacteria strains from diverse soil ecosystem of Tamanrasset town, and describe the morphological diversity of the isolates. We applied physical and chemical treatment to enhance the isolation of Actinobacteria strains on different free antibiotics culture media. Actinobacteria isolates were characterized based on macroscopic and microscopic observation. In addition, the preliminary screening for antibacterial activity was carried out by the Cross streak method on three culture media (Glycerol yeast extract “GYE”, Glycerol soil extract “GSE” and Mueller Hinton “MH”) against six clinical bacteria strains viz., Staphylococcus aureus, Escherichia coli, Enterococcus faecal, Enterobacter cloacae and two strains of Streptococcus spp. Ten (10) different Actinobacteria isolates, exhibit a distinct morphology were obtained. Sixty-seven percent (67%) of the tested isolates showed at least one antibacterial activity against indicator clinical bacteria, the tested Actinobacteria A1, A6, and A8 have the highest expression on MH medium, whereas A6 has the largest range of antibacterial activity against all indicator clinical bacteria in MH medium. However, in some cases, the change of media influence positively the antibacterial activity. The present study revealed that Tamanrasset soil is rich in Actinobacteria strains, this finding of Actinobacteria can be of importance for further investigation towards obtaining broad-spectrum bioactive compound for medical purpose.