IN SILICO RESEARCH OF GENES AND ENCODED PROTEINS FOR SALT STRESS TOLERANCE IN THE LEGUMINOUS Vicia faba L.

Abstract

Abiotic stresses affect plant productivity and crop yield, especially legumes. Identifying stress tolerance genes and proteins is important for improving stress tolerance in plants. In addition to the in vitro and in vivo approach, the in silico approach, using bioinformatics tools, represents a predictive and complementary demarche compared to other approaches. In this study, proteins and genes for salt stress tolerance were identified in silico in Vicia faba L., using molecular databases (NCBI, EMBL-EBI and UniProt) and the Blast tool. The analysis made it possible to identify the stress proteins and their coding genes by looking for similarities of function in the model species Arabidopsis thaliana (L.) Heynh and Medicago truncatula Gaertner. The results showed quite significant sequence similarities (E-value = 0) and a high score (high percentage of identity). For example, the proteins "Aabscisic acid activated protein kinase "," Serine/ threonine protein kinase; SRK2I‖ and Serine/ threonine protein kinase SRK2E‖ for salt stress.