INSECTICIDAL ACTIVITY OF ETHYL ACETATE EXTRACT OF Borago officinalis L. (BORAGINACEAE) AGAINST Ctenocephalides felis And Archaeopsylla erinacei (SIPHONOPTERA, PULLICIDAE)

Abstract

Vector-borne diseases account for more than 17% of infectious diseases, and cause more than one million deaths each year. However, the misuse of chemical insecticides has resulted in insect resistance. This work concerns the evaluation of the insecticidal activity of the ethyl acetate extract of Borage (Borago officinalis L.), a plant widely distributed in northern Algeria, on the cat flea Ctenocephalides felis and the flea of the Hedgehog Archaeopsylla erinacei, carriers of serious vector diseases. For that, adult flea specimens of C. felis and A. erinacei were exposed to a series of concentrations of plant extracts. (50 mg / ml, 25 mg / ml, 12.5 mg / ml and 6.25 mg / ml) using pre-impregnated wattman paper strips. The plant extract was obtained by fractionation using appropriate solvents; it was then characterized by high performance thin layer chromatography (HPTLC). The extraction yield was 4.87% with a flavonoid concentration of 120.99 ± 0.81 μgeq Q / g extract. Chromatographic characterization by HTPLC reveals the presence of rutin, rhamnetin and cyanidin-3-glucoside in this extract. The results obtained show that the ethyl acetate extract of Borage greatly reduces the population of the two species of flea A. erinacei and C. felis after 48 hours of contact. The LC50 values were 9.74 mg / ml and 11.24 mg / ml respectively. The mortality kinetics results show TL50 values of 7.29h and 8.51h on the same species respectively.