A comparative study of the phytochemical, antioxidant and anti-inflammatory properties of ethyl acetate extract of Asphodelus tenuifolius Cav recovered by different extraction techniques

Abstract

In this study, phytochemical, in vitro antioxidant activities and anti-inflammatory of bioactive components of Asphodelus tenuifolius Cav extracts by classical extraction method (CE), Soxhlet extraction (SE), ultrasonic extraction (UE) and microwave extraction (ME) were investigated. The total phenolic, flavanoids, condensed tannins and anthocyanin content were determined by a spectrophotometric method. β-Carotene, ABTS, and FRAP radical-scavenging were applied to test the antioxidant activities. The results indicated that the classical method extraction exhibited the strongest antioxidant activities. The contents of polyphenols in the CE were significantly higher than those by other extraction methods, which was possibly responsible for the higher antioxidant activities of the Asphodelus tenuifolius Cav by CE. From the results we can draw the conclusion that not only the more bioactive components are obtained but also the extract has better free radical and reactive oxygen species scavenging activities through classical extraction method. These findings further illustrate that classical extraction has a bright prospect for extracting active ingredients from plant materials. The study concludes that Asphodelus tenuifolius Cav possess diverse therapeutic potentials which might be useful in development of drugs or their precursors