Synthesis and Study of the Antioxidant and Antimutagenic Activity of Some Porphyrins

Abstract

This thesis proposes a comprehensive study aimed at first evaluating the antioxidant properties of selected porphyrin derivatives, including nickel tetraphenyl-porphyrin (NiTPPH2), meso-tetraphenyl-porphyrin (TbiPPH2), meso-methyl tetraphenyl-porphyrin (TPPH2 (o-methyl)), and zinc tetraphenyl-porphyrin (ZnTPPH2 (p-methyl)), by assessing their antioxidant activities using electrochemical and spectroscopic means. Additionally, interaction parameters between these derivatives and DPPH radicals as well as the superoxide anion were calculated to elucidate their potential mechanisms of action. Secondly, the study aims to evaluate the antimutagenic properties by conducting experimental evaluations to determine the binding free energy and binding constants of these derivatives with DNA and BSA. The methodologies employed included electrochemical techniques, such as cyclic voltammetry, and spectroscopic techniques, particularly UV spectral analysis. The validity of the results was confirmed through molecular docking using AutoDock tools. This thesis proposes a comprehensive study aimed at first evaluating the antioxidant properties of selected porphyrin derivatives, including nickel tetraphenyl-porphyrin (NiTPPH2), meso-tetraphenyl-porphyrin (TbiPPH2), meso-methyl tetraphenyl-porphyrin (TPPH2 (o-methyl)), and zinc tetraphenyl-porphyrin (ZnTPPH2 (p-methyl)), by assessing their antioxidant activities using electrochemical and spectroscopic means. Additionally, interaction parameters between these derivatives and DPPH radicals as well as the superoxide anion were calculated to elucidate their potential mechanisms of action. Secondly, the study aims to evaluate the antimutagenic properties by conducting experimental evaluations to determine the binding free energy and binding constants of these derivatives with DNA and BSA. The methodologies employed included electrochemical techniques, such as cyclic voltammetry, and spectroscopic techniques, particularly UV spectral analysis. The validity of the results was confirmed through molecular docking using AutoDock tools. This thesis proposes a comprehensive study aimed at first evaluating the antioxidant properties of selected porphyrin derivatives, including nickel tetraphenyl-porphyrin (NiTPPH2), meso-tetraphenyl-porphyrin (TbiPPH2), meso-methyl tetraphenyl-porphyrin (TPPH2 (o-methyl)), and zinc tetraphenyl-porphyrin (ZnTPPH2 (p-methyl)), by assessing their antioxidant activities using electrochemical and spectroscopic means. Additionally, interaction parameters between these derivatives and DPPH radicals as well as the superoxide anion were calculated to elucidate their potential mechanisms of action. Secondly, the study aims to evaluate the antimutagenic properties by conducting experimental evaluations to determine the binding free energy and binding constants of these derivatives with DNA and BSA. The methodologies employed included electrochemical techniques, such as cyclic voltammetry, and spectroscopic techniques, particularly UV spectral analysis. The validity of the results was confirmed through molecular docking using AutoDock tools.

Cette thèse propose une étude exhaustive visant d'abord à évaluer les propriétés antioxydantes de certains dérivés de porphyrine sélectionnés, à savoir tétraphénylporphyrine de nickel (NiTPPH2), la tétraphénylporphyrine de méso (TbiPPH2), la tétraphénylporphyrine de mésométhyl (TPPH2 (o-méthyl)) et la tétraphénylporphyrine de zinc (ZnTPPH2 (p-méthyl)), en évaluant leurs activités antioxydantes par des moyens électrochimiques. De plus, les paramètres d'interaction entre ces dérivés et les radicaux DPPH ainsi que l'anion superoxyde ont été calculés pour élucider leurs mécanismes d'action potentiels. Deuxièmement, l'étude vise à évaluer les propriétés antimutagènes en effectuant des évaluations expérimentales pour déterminer l'énergie libre de liaison et les constantes de liaison de ces dérivés avec l'ADN et la BSA. Les méthodologies utilisées comprenaient des techniques électrochimiques, telles que la voltamétrie cyclique, et des techniques spectroscopiques, notamment l'analyse spectrale UV. La validité des résultats a été vérifiée par l'amarrage moléculaire utilisant l’outil AutoDock

تقترح هذه الأطروحة إجراء دراسة شاملة تهدف أولاً إلى تقييم خصائص مضادات الأكسدة لبعض مشتقات البورفيرين المختارة، وهي النيكل رباعي فينيل بورفيرين , ميزو رباعي ثنائي فينيل بورفيرين. ميزو رباعي ميثو فينيل بورفيرين, ورباعي ميثو فينيل بورفيرين الزنك, و ذلك من خلال تقييم أنشطتها المضادة للأكسدة بالوسائل الكهروكيميائية والتقنيات الطيفية. علاوة على ذلك، تم حساب معاملات التفاعل بين هذه المشتقات وجذورDPPH وكذلك أنيون الأكسيد الفائق لتوضيح آليات عملها المحتملة. ثانياً، تهدف الدراسة إلى تقييم الخواص المضادة للطفرات من خلال إجراء تقييمات تجريبية لتحديد طاقة الارتباط الحرة وثوابت الارتباط لهذه المشتقات معDNA و BSA . وشملت المنهجيات المستخدمة التقنيات الكهروكيميائية، مثل قياس الجهد الدوري، والتقنيات الطيفية، بما في ذلك التحليل الطيفي للأشعة فوق البنفسجية. وتم التحقق من صحة النتائج عن طريق الالتحام الجزيئي باستخدام أدوات .AutoDock